DNA methylation status of TBX20 in patients with tetralogy of Fallot

BMC Medical Genomics

Table 2 Primer sequences, position, product length and CpG units used for MassArray quantitative methylation analysis

Gene	Forward primer (5′ → 3′)^a	Reverse primer (5′ → 3′)^b	Distance	Product	No of CpG’s	Coverage
Gene	Forward primer (5′ → 3′)^a	Reverse primer (5′ → 3′)^b	(bp)^c	Length (bp)		(CpG units)
TBX20_M1	TTTGAGTGTGTATGTTAGTTTGAGTTT	CTCCTATTTTCCCTAAAAAAACCCT	−945~ − 635	311	23	22
TBX20_M2	GTGAGATTAGGTGGGGATGTTTAT	TCCTTCCTCCCTCTAAAACTAAAAA	− 214~167	382	42	32

ISSN: 1755-8794