Normalization methods | Expression ratio* | Bias** | Variance** | ||||||
---|---|---|---|---|---|---|---|---|---|
0–0.01 (%) | 0.01–0.05 (%) | 0.05–0.20 (%) | 0.20–0.40 (%) | 0.40–0.60 (%) | 0.60–0.80 (%) | 0.80–1.0 (%) | |||
TC | 396 (41.60) | 473 (49.68) | 78 (8.19) | 1 (0.11) | 1 (0.11) | 0 (0) | 3 (0.32) | 0.74 | 0.55 |
UQ | 216 (22.69) | 612 (64.29) | 115 (12.08) | 3 (0.32) | 4 (0.42) | 1 (0.11) | 1 (0.11) | 0.45 | 0.21 |
Median | 157 (16.49) | 643 (67.54) | 142 (14.92) | 7 (0.74) | 2 (0.21) | 0 (0) | 1 (0.11) | 0.45 | 0.22 |
Quantile | 125 (13.13) | 655 (68.80) | 161 (16.91) | 6 (0.63) | 3 (0.32) | 1 (0.11) | 1 (0.11) | 0.42 | 0.18 |
TMM | 236 (24.79) | 599 (62.92) | 108 (11.34) | 4 (0.42) | 4 (0.42) | 0 (0) | 1 (0.11) | 0.47 | 0.23 |
DESeq | 231 (24.26) | 610 (64.08) | 104 (10.89) | 4 (0.42) | 2 (0.21) | 0 (0) | 1 (0.11) | 0.43 | 0.19 |
TPM | 157 (16.49) | 643 (67.54) | 142 (14.92) | 7 (0.74) | 2 (0.21) | 0 (0) | 1 (0.11) | 0.45 | 0.22 |
RPKM | 603 (63.34) | 319 (33.51) | 26 (2.73) | 2 (0.21) | 0 (0) | 0 (0) | 2 (0.21) | 1.04 | 1.03 |
Lib_size | 202 (21.22) | 617 (64.81) | 123 (12.92) | 7 (0.74) | 2 (0.21) | 0 (0) | 1 (0.11) | 0.43 | 0.20 |