Fig. 1

Overview of dataflow in MySeq. The MySeq single-page web application performs personal genome analyses in the user’s web browser. (1) MySeq components query a locally stored or remotely available VCF file by genomic coordinates. (2) Internally MySeq uses the Tabix index to fetch and parse only the portion of the file containing variants in the query region. (3) MySeq further analyzes the VCF records entirely in the browser (e.g. displays the genotypes to the user, performs ancestry analysis, etc.). Optionally MySeq can utilize the publicly available MyVariant.info and MyGene.info APIs [37] to annotate variants or translate gene symbols or rsIDs to genomic coordinates for queries (e.g. query for all variants in BRCA1), but does not send any genotypes to a remote server