Fig. 3
From: Genome scale analysis of pathogenic variants targetable for single base editing
Scheme of searching for potential targets for base editors. First the script searches for PAM near (yellow) the mutation based on the characteristics of the individual editor: PAM sequence and the editing window, in which the targeted nucleotide should fit (a). If the PAM is found in the necessary area, the script fixes its coordinates (green) and analyses the editing window (orange) to select only the window without other cytosine (or adenine) residues to reduce the risk of unwanted editing close to zero (b). a, search for PAM sequences around the mutation; X – beginning of the editing window, Y – end of the editing window. b, Analysis of the DNA sequence in the editing window around the selected mutation