Table 1 Quantitative real-time primers
No. | primer | position | Sequence(5′-3′) |
|---|---|---|---|
1 | SHANK3-1F | Intro 1 | TGACCAGAGGCTGTTTTGAG |
2 | SHANK3-1R | Intro 1 | CAGCAGATCCACCTCGACC |
3 | SHANK3-8F | Intro 8 | CCTGCGCACGCCATGT |
4 | SHANK3-8R | Intro 8 | GAGACCATCCGAGCACAACA |
5 | SHANK3-11F | Intro 11 | CACCTGTGTAGTGATGGGCT |
6 | SHANK3-11R | Intro 11 | CTCTCCACCTAACACGCTCC |
7 | SHANK3-17F | Exon 17 | CGCCTCGTCATGAAGGTTGT |
8 | SHANK3-17R | Exon 17 | CGAGCCCCGTCCTCTTCT |
9 | SHANK3-22F | Exon 22 | GGAGAGCGGGGAACTCACT |
10 | SHANK3-22R | Exon 22 | CTGTCCGAGGACTGCTTCAG |
11 | SHANK3-23F | Exon 23 | ACTCATCCTTCCGCCAACAG |
12 | SHANK3-23R | Exon 23 | CCCACAGGTGAGTGTGAGAC |
13 | FBLN1-4F | Exon 4 | AGAGCTGCGAGTACAGCCT |
14 | FBLN1-4R | Exon 4 | CGACATCCAAATCTCCGGTCT |
15 | FBLN1-11F | Intro11 | GGACCTCTGTCTCTCCGAGT |
16 | FBLN1-11R | Intro11 | ACCGCTCAGAGCATCATACG |
17 | FBLN1-14F | Intro14 | TCCTCCCATGAGGGACTCAG |
18 | FBLN1-14R | Intro14 | CACAGCCTTGGCCTGAAAAC |
19 | GAPDH-8F | Intro 8 | ACTGGCTCTTAAAAAGTGCAGGGT |
20 | GAPDH-8R | Intro 8 | TTGCTGTAGCCAAATTCGTTGTC |