Fig. 4

Changes in the expression of RNA Seq single-cell signatures with gestational age. For each single-cell signature, the expression of member genes (based on Tsang et al. [105]) was transformed into Z-scores and averaged in each AF sample. A Robust Locally Weighted Regression and Smoothing Scatterplots (LOESS) model fit through the Z-scores as a function of gestational age is shown using lines (see Fig. S3 for individual values). Single-cell signature trends are set to have the same value at 16 weeks of gestation. AF, amniotic fluid