Fig. 3
From: Detection of gene fusions using targeted next-generation sequencing: a comparative evaluation
Representative examples of the ETV6-NTRK3 fusion in sample 3 detected by all five assays and their respective data analysis tools. Metrics such as quality control scores, in-frame status or filter thresholds were plotted when available. In cases where the same fusion was identified more than once within the same sample, a unique numbering scheme was added at the end of the name to differentiate the candidate fusions. For all the kits, the putative detected fusions were arranged in decreasing order based on the number of fusion-supporting reads, with the exception of QIAseq RNAscan, where the software already provides the results with an ordering scheme with the most likely fusion being called “Fusion 1”. The expected fusion for each sample was highlighted in bold. (a) Archer FusionPlex Lung Panel (Archer DX), (b) QIAseq Targeted RNAscan Custom Panel (Qiagen), (c) Oncomine Focus Assay (Thermo Fisher Scientific), (d) TruSight Tumor 170 Assay (Illumina) and (e) SureSelect XT HS Custom Panel (Agilent)