Table 2 cDNA synthesis specific stem-loop primers and real-time PCR primer sequences
| Â | Noncoding-RNAs and their accession numbers | Sequences | |
|---|---|---|---|
cDNA synthesis reaction | MiR-3163-5p NIMAT 0015037 | MiR-3163 (STL) | 5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGTCTTAC-3′ |
Mir-513c-5p NIMAT 0022728 | Mir-513c (STL) | 5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACATAAAC-3′ | |
RNU6 NR_003027.2 | U6 (STL) | 5′-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAAATAT-3′ | |
Real time PCR reaction | MiR-3163-5p NIMAT 0015037 | Mir-3163 (F) | 5′-AGGGTATAAAATGAGGGCAGTAAGAC-3′ |
Mir-513c-5p NIMAT 0022728 | Mir-513c (F) | 5′-GGGTTCTCAAGGAGGTGTCG-3′ | |
MiR-3163 and Mir-513c | Common (R) | 5′-GTGCAGGGTCCGAGGT-3′ | |
RNU6 NR_003027.2 | U6 (F) | 5′-GCTTCGGCAGCACATATACTAAAAT-3′ | |
U6 (R) | 5′-CGCTTCACGAATTTGCGTGTCAT-3′ | ||