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Fig. 6 | BMC Medical Genomics

Fig. 6

From: Identification and validation of pivotal genes related to age-related meniscus degeneration based on gene expression profiling analysis and in vivo and in vitro models detection

Fig. 6

The expressions of Hub genes in the mouse meniscus cells. A SA-β-gal staining of mouse senescent meniscus cells induced by IL-1β (10 ng/ml) for 48 h. Scale bar: 500 μm. B, D Apoptotic analysis detected by Annexin V/7AAD after IL-1β disposition. C Detection of the expressions of hub genes in senescent meniscus cells via qRT-PCR. We normalized the gene expression levels to GAPDH. E Immunofluorescence analysis of hub genes after treating meniscus cells with10ng/ml IL-1β for 48 h. Scale bars, 50 μm. RRM2 ribonucleotide reductase regulatory subunit M2; AURKB aurora kinase B; CDK1 cyclin dependent kinase 1; TIMP1 tissue inhibitor of metalloproteinases 1; GAPDH Glyceraldehyde 3-phosphate dehydrogenase. n = 6 per group, mean ± S.E.M., *P < 0.05, **P < 0.01 in comparison with the control group

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BMC Medical Genomics

ISSN: 1755-8794

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