Table 2 Primers used for PCR amplification and sequencing of CV-A5 genome
From: Emergence of a novel recombinant of CV-A5 in HFMD epidemics in Xiangyang, China
Name | Primer sequence (5′–3′) | Position (nt) |
|---|---|---|
A5 1F | TTAAAACAGCCTGTGGGTTGTACC | 1–24 |
A5 6F | GCAATCCACTATGGTAGGACAACT | 1998–2021 |
A5 9F | GCACCTTTTCAGTGAGATTCGTT | 3128–3250 |
A5 9R | ACCTACTGCAAGCATGAGATG | 3630–3610 |
A5 13R | AACCCAGCAAAGAGCTTGTAGATA | 4825–4806 |
A5 20F | AAGAGAGTCTACGCCCTGGAG | 4366–4386 |
A5 23R | TTGACCCCTTGTTCATCCACTAAT | 5588–5565 |
A5 24F | TCACTGCTGAGAAGGAATATCAGG | 5407–5430 |
A5 24R | CCAAAATGTATCTGGATTGCACCC | 6585–6562 |
A5 polyA | AGTCAAGTTACATAGTAGGCTACAGTAACTGCTTTTTTTTTTTTTTTTTT | 7370–7419 |