Table 2 The characteristics and sequences of primers used for detection of genes polymorphisms
| Gene (polymorphism) | Primer sequence (5′ → 3′) | Products size: allele type |
|---|---|---|
| CATSPER1 (rs2845570) |
Forward outer: TCCAGCATGACGGTGTTGAGGCAGA Reverse outer: ATATTCTTCTGCATCTACGTGGTGG Forward inner: TTCTTGCGGGTCCGCTGGAGCCG Reverse inner: TCTGGCCCTGTTCCTTTCAGCCGGCA |
323 bp: allele G (A) 473 bp: – 198 bp: allele T (B) |
| SPATA16 (rs1515442) |
Forward outer: TAACATCCTGGAAATGTCACAAGAG Reverse outer: TTCTTTAATCCCATACCTCAAGTGC Forward inner: ATGAAGTTGGTCTACATTGATGAAA Reverse inner: CTACAAACTCATAGCGAACACCCAC |
256 bp: allele T (A) 515 bp: – 308 bp: allele C (B) |
| TEX11 (rs143246552) |
Forward outer: ATAGATTCCAATCAGCATTAGTAACATC Reverse outer: AGGATTCAATATTTTCTCCAATATTCCC Forward inner: TCACTCTCAACAGTAATCTTCTCCAT Reverse inner: CCCTGAGGCTGACTTGACCG |
348 bp: allele T (A) 563 bp: – 470 bp: allele C (B) |