Inter-assay variability of next-generation sequencing-based gene panels

BMC Medical Genomics

Table 1 Clinical and preanalytical characteristics of 30 patients

Group	No	Age	Sex	Cancer type	Histology	Stage	Concentration of library DNA (nM)	Q-value
FF	1	45	M	Pancreas	Adenocarcinoma	IV	8.2	0.85
	2	57	M	Colorectal	Adenocarcinoma	IV	16.2	0.82
	3	56	F	CUP	Adenocarcinoma	IV	18.0	1.00
	4	52	M	Biliary tract	Adenocarcinoma	IV	14.4	0.60
	5	56	M	Pancreas	Adenocarcinoma	IV	13.0	0.79
	6	72	M	Colorectal	Adenocarcinoma	IV	18.5	0.72
	7	82	M	Pancreas	Adenocarcinoma	IV	5.8	0.85
	8	55	F	Esophagus	Squamous cell carcinoma	IV	30.7	1.43
	9	61	F	Pancreas	Neuroendocrine tumor	IV	21.9	1.23
	10	58	F	Biliary tract	Adenocarcinoma	IV	25.1	0.89
FFPE-H	11	47	F	Pancreas	Adenocarcinoma	IV	13.2	1.32
	12	62	F	CUP	Adenocarcinoma	IV	28.5	0.29
	13	72	F	CUP	Squamous cell carcinoma	IV	36.9	0.20
	14	69	M	CUP	Adenocarcinoma	IV	12.1	0.36
	15	64	F	NSCLC	Adenocarcinoma	IV	12.8	0.23
	16	65	F	Gastric	Adenocarcinoma	IV	7.4	1.06
	17	73	M	NSCLC	Adenocarcinoma	IV	4.9	0.43
	18	57	F	Pancreas	Adenocarcinoma	IV	8.1	0.26
	19	73	M	Esophagus	Squamous cell carcinoma	IV	8.2	0.36
	20	69	F	Sarcoma	Leiomyosarcoma	IV	6.1	0.44
FFPE-L	21	36	F	NSCLC	Adenocarcinoma	IV	3.4	0.22
	22	39	F	Colorectal	Adenocarcinoma	IV	1.0	0.21
	23	64	F	Breast	Adenocarcinoma	IV	1.0	0.44
	24	57	M	Esophagus	Squamous cell carcinoma	IV	1.6	0.14
	25	52	F	Biliary tract	Adenocarcinoma	IV	0.2	0.11
	26	61	F	Pancreas	Adenocarcinoma	IV	2.3	0.29
	27	68	M	Biliary tract	Adenocarcinoma	IV	4.2	0.57
	28	66	M	Colorectal	Adenocarcinoma	IV	1.4	0.07
	29	65	M	Gastric	Adenocarcinoma	IV	1.8	0.34
	30	73	M	Bladder	Urothelial carcinoma	IV	4.0	0.27

M male, F female, CUP cancer of unknown primary, NSCLC non-small cell lung cancer

ISSN: 1755-8794