Fig. 3

Expression of the wild-type and mutant kAE1 proteins. a Western blot analysis of the wild-type and mutant kAE1 proteins and endogenously expressed GAPDH in HEK293T cells. Images of the blots cropped from different parts of the same blot were separated by dividing lines. Full-length blots are presented in Additional file 1: Fig. S2. b Analysis of cell surface expression of the wild-type and the mutant kAE1 SAO and T444N proteins by flow cytometry. c Analysis of cell surface expression in co-expressions of the wild-type and the mutant kAE1 SAO or T444N proteins or the mutant kAE1 SAO and T444N proteins in HEK293T cells by flow cytometry. The transfected cells were stained with mouse anti-Myc antibody, followed by AlexaFluor 488-conjugated anti-mouse antibody. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test (*p < 0.05, **p < 0.01, ***p < 0.001)