Fig. 1
From: Disruption of DDX53 coding sequence has limited impact on iPSC-derived human NGN2 neurons
Outline of CRISPR-Cas9 experimental approach to generate DDX53-3TC. A) The DDX53 target allele locus with CRISPR/Cas9 approach to insert 3TCs tag into DDX53 via ssODN template. B) Insertion of composite tag to make a DDX53-3TC clone was identified by absolute quantification of tag or wild type alleles. C) Sequence of DNA in DDX53-3TC iPSC line confirms integration of composite tag within DDX53