Fig. 2
From: Disruption of DDX53 coding sequence has limited impact on iPSC-derived human NGN2 neurons
Characterization of human DDX53-3TC iPSCs. Blue droplets are FAM-positive droplets (WT), brown are double-positive droplets, gray are negative droplets, and green are VIC-positive droplets (mutant). (Progress of mutant allele frequency from the highest signal wells following clonal selection for 19-2-DDX53-3TC. A) and 50B-DDX53-3TC. B) Expression levels of DDX53 gene in control lines, and testis (positive control) as a fraction of that from an internal control, TBP (N = 4/8 for 19-2 DDX53; N = 3/6 for 50B DDX53; N = 1/3 for testis). Numbers with each bar indicate the number of biological replicates/number of technical replicates. The y-axis shows the ratio of DDX53 transcript to TBP transcript in different samples. C) ***p < 0.001, one way ANOVA with Tukey multiple comparisons test