Fig. 5

Changed STC2 expression in osteosarcoma comparing to control samples

CCLE prediction of STC2 gene expression in broad spectrum human cancer cell lines, the number in abscissa parentheses represent count of cell lines involved in the analysis. (B) Oncomine compares the expression of STC2 in different osteosarcoma subtypes. (C) Human Protein Atlas predicts STC2 expresses in perinuclear endoplasmic reticulum or to be secreted extracellular. (D) IHC experiment using local hospital patients samples reveals STC2 expresses in perinuclear pattern as predicted in breast cancer which is suggested by the antibody operating instruction to be staining control cancer. (E) IHC experiment reveals that as opposed to the control breast cancer, most of the STC2 expresses in osteosarcoma as nuclear staining, and the expression was higher in cancer comparing to (F) control bone samples. Left corner of (D, E, F) was the original sample slice used for slide scanning. Scale bars, 20 μm. Magnification, 200x and 400x in the original images (left) and magnification images (right) for Figure D and E, meanwhile 50x and 400x for figure F respectively