Fig. 4

Alteration of annexin A1 localization in the stria vascularis of the Slc26a4 mice. A: Rank-ordered pH-dependent genes based on ROC AUC score. B-C: Representative images showing cochlea section with ANXA1 (green), pendrin (red), and nuclei (cyan). Scale bar: 50 μm. (oC, organ of Corti; OS, outer sulcus; RC, root cells; SC, spindle cells; SP, spiral prominence; SV, stria vascularis). D: High magnification of B-C. Note: In Slc26a4^+/+ mice, ANXA1 expression co-localizes with pendrin in the apical membranes of the SC. In contrast, in Slc26a4^−/− mice, without pendrin expression, the area of the ANXA1 positive cells is expanded. Scale bar: 20 μm. E: Box plot showing the relative fluorescence intensity of pendrin and ANXA1 in SCs. (***, p < 0.0005). In Slc26a4^−/− mice, pendrin is absent. Note: No significant difference in ANXA1 intensity was observed between Slc26a4^+/+ and Slc26a4^−/− mice. F: High magnification of images of the SV area represented in B-C. ANXA1 (green), F-actin (red), and nuclei (cyan). SV thickness of Slc26a4^+/+ and Slc26a4^−/− measured at the widest portion (site of measurement, ↔). Scale bar: 20 μm. G: Box plot showing the relative fluorescence intensity of ANXA1 in SV cells and SV thickness (***, p < 0.0005). Note: ANXA1 is absent in the SV of Slc26a4^+/+ mice but is present in Slc26a4^−/− mice. These results may reflect the alteration of the exocytosis signaling pathway