Fig. 6
LSM2 expression profile and transfection in cell lines
A. Relative LSM2 expression was investigated in five cutaneous melanoma cell lines and compared with that in the normal human epidermal melanocyte cell line, HEMa-LP. LSM2 expression was explored using RT-PCR and normalized to GAPDH expression
B, C. Western blot analysis of LSM2 protein expression in A375 or A2058 cells, negative control cells, and cells transfected with siRNA-1, siRNA-2, and siRNA-3.
D, E. LSM2 was silenced by siRNA-1, siRNA-2, and siRNA-3 in A375 or A2058 cells. LSM2 expression was examined using RT-PCR.