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Fig. 8 | BMC Medical Genomics

Fig. 8

From: Identification of necroptosis-related gene TRAF5 as potential target of diagnosing atherosclerosis and assessing its stability

Fig. 8

TRAF5 knockdown enhances necroptosis and attenuates the proliferation of VSMCs induced by oxidized-low density lipoprotein (ox-LDL). A RT-qPCR to confirming TRAF5 expression in VSMCs treated with TRAF5 siRNAs. B-E Western blotting detecting the levels of RIPK3, MLKL, and p-MLKL in ox-LDL-induced VSMCs with TRAF5 knockdown (n = 3, normalized to GAPDH). F Cell counting kit 8 assay (CCK8) evaluating cell proliferation (n = 3). G, H EdU assay evaluating cell proliferation (n = 3). I, J Necroptosis was detected by flow cytometry after Annexin V/PI staining. Annexin V-/PI- represents living cells, Annexin V + /PI- represents early necroptotic cells, and Annexin V + /PI + represents necroptotic cells (n = 3). (ns: no significance; *p < 0.05; **p < 0.01; ***p < 0.001)

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BMC Medical Genomics

ISSN: 1755-8794

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