Fig. 2

MiR-455-3p inhibits cell proliferation and EMT but promotes apoptosis in GC (A - D) The effects of miR-455-3p on proliferation in HGC-27 and AGS cells transfected with miR-455-3p mimics or inhibitors via EdU incorporation assay(Magnification 200×) (A, B) and colony formation assays (C, D). E, F of Apoptosis in HGC-27 and AGS transfected with miR-455-3p mimics or inhibitors assessed by Flow cytometry. The total events shown in the lower right-hand and upper right-hand quadrants are apoptotic cells. G-J Western blotsshowing protein levels of Bcl-2, Caspas-3, Bax, E-cadherin N-cadherin and Snail in HGC-27 and AGS cells transfected with miR-455-3p mimics or inhibitors. β-actinis a loading control. Data are representative of three independent experiments. The statistical significance was evaluated using Student’s t tests. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001