Fig. 3

ARMC8 is a direct target of miR-455-3p by binding to 3’ UTR region in GC 9 (A) Online databases were used to predict target genes, followed by intersection analyses. ARMC8 have a high target scores in the miRDB database. B The ARMC8 3’UTR contained potential binding sites for miR-455-3p. C, D HGC-27 and AGS cells were co-transfected with miR-455-3p mimics or inhibitors and wild-type (WT) or mutant (MT) ARM8 3’UTR plasmids. Dual luciferase activity was determined after 48 h. E, F Analyses of ARMC8 expression in HGC-27 and AGS cells transfected with miR-455-3p mimic. β-actin is a loading control. G Expression level of ARMC8 in GC tissues compared with adjacent non-tumor tissues in The Cancer Genome Atlas dataset. H The relationship between ARMC8 and miR-455-3p are representative. I Expression of ARMC8 in human GC tissues and adjacent tissues. J, K Expression of ARMC8 in GES-1, HGC-27, and AGS cells was detected by qPCR and western blotting. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001