Fig. 4

MiR-455-3p functions as a tumor suppressor via targeting ARMC8 in GC (A, B) Western blots showing ARMC8 expression in HGC-27 and AGS cells transfected with miR-455-3p mimic or miR-455-3p mimic plus ARMC8 expression vector (Magnification 200×) (C, D) EdU incorporation assay data showing cell proliferation of HGC-27 and AGS cells transfected with miR-455-3p mimic or miR-455-3p mimic plus ARMC8 expression vector. E, F Flow cytometry data showing apoptosis in HGC-27 and AGS cells transfected with miR-455-3p mimic or miR-455-3p mimic plus ARMC8. Total events shown in the lower right-hand and upper right-hand quadrants are apoptotic cells. G, H Protein levels of E-cadherin N-cadherin and Snail were determined by using western blotting. Data are presented as the mean ± SD. *p < 0.05, **p< 0.01, ***p < 0.001