Table 1 primer sequences used for PiZ and PiS AAT genotypes
From: Association of A1AT genetic polymorphism and NSCLC: a case- control study in Egyptian population
Mutation | Primer sequence | Size (bp) |
|---|---|---|
Pi Z allele rs8004738 | Sense Z: 5´GCTGTGCTGACCATCGACA 3´ | 288 bp |
Anti-sense Z/ non-Z:5´ CCAGGGATTTACAGATCACATGC-3´ | ||
Sense non-Z:5´-GCTGTGCTGACCATCGACG-3´ | 288 bp | |
Anti-sense Z/ non-Z:5´-CCAGGGATTTACAGATCACATGC-3´ | ||
Pi S allele rs17580 | Anti-sense S: 5´-ATGATATCGTGGGTGAGTTCATTTA-3´ | 221 bp |
Sense S/ non-S 5´-GAAGTCAAGGACACCGAGGA- 3´ | ||
Anti-sense non-S:5´-ATGATATCGTGGGTGAGTTCATTTT-3´ | 221 bp | |
Sense S/ non-S 5´-GAAGTCAAGGACACCGAGGA- 3´ |