Fig. 2

Genetic and transcriptional alterations of ERGs in GBM. A Distribution of expression of ERGs between normal and GBM. B Mutation frequency of 20 ERGs in the TCGA cohort of 390 GBM patients. C Copy number variation (CNV) of 20 ERGs in TCGA-GBM. D Localization of 20ERGs in chromosomal regions. E Network plot showing the correlation between the 20 ERGs. Red connecting lines indicate positive correlations, while blue indicates negative correlations. F TIMD4 was highly expressed in GBM cell lines compared to normal human astrocyte NHA cell lines. G RT-qPCR was performed to detect the relative expression of TIMD4 in GBM cells transfected with si-RNAs or negative control (NC). H CCK8 assay showed that SW1783 cells with reduced TIMD4 expression had significantly reduced proliferative capacity compared to the NC group. I, J Transwell assay showed that down-regulation of TIMD4 expression inhibited the migration and invasion ability of SW1783 cells. All data are expressed as mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001