Fig. 4

Cellular developmental trajectories and gene regulatory networks of H5N6-associated ILC subpopulations. (A) Pseudotime analysis showing pseudotime values and pseudotime trajectories of ILC subpopulations from control to disease progression. (B) Overlayed pie charts representing the proportion of control and H5N6-infected individuals in each subpopulation. (C) Co-expression modules of transcription factors in ILC subpopulations. Left: Identification of regulator modules based on the connection specificity index matrix of regulators. Middle: representative transcription factors and their binding patterns in the modules. Right panel: cell subpopulations in which transcription factors are located. (D) Series of scatter plots showing transcription factors regulating ILC subpopulations. UMAP, Uniform Manifold Approximation and Projection; ILC, Innate lymphoid cell