Fig. 6

Cellular developmental trajectories and gene regulatory networks of H5N6-associated CD4⁺T cell subpopulations. (A) Pseudotime analysis demonstrating pseudotime values and pseudotime trajectories of CD4⁺ T cell subpopulations from control to disease progression. (B) Overlayed pie charts representing the proportion of control and H5N6-infected individuals in each subpopulation. (C) Co-expression modules of transcription factors in CD4⁺ T cell subpopulations. Left: identification of regulator modules based on the connection specificity index matrix of regulators. Middle: representative transcription factors and their binding patterns in the modules. Right panel: cell subpopulations where transcription factors are located. (D) Series of scatter plots showing transcription factors regulating CD4⁺ T cell subpopulations. UMAP, Uniform Manifold Approximation and Projection