Fig. 4

Positions of guide RNAs designed for the target, here the TRPA1 promoter. Positions of guide RNAs are depicted relative to the target region on chromosome 8. Arrows mark outmost GRCh38 positions. The guide RNAs are numbered (compare Tables 1 and 3) and are depicted as boxes; PAM motifs are indicated by filled squares. The black coloring indicates guide RNAs with a PAM motif pointing towards the target, whereas guideRNAs with a PAM motif facing away from the target (and therefore interfering/ disturbing) are colored in grey. The guide RNAs yielding the highest number of calls per site (# 4 and # 9) are printed in bold