Fig. 5

Methylation rates of the TRPA1 promoter determined via nanopore sequencing in relation to the number of calls per site. Data based on 12 measurements of the same DNA sample. The red line marks the methylation rate measured with the highest number of calls per site per CpG, which is presumably the most accurate value. Several flow cells and different guide RNA strategies for Cas-mediated PCR-free enrichment were used leading to varying numbers of calls per site