Table 1 Guide RNAs used for establishing the Cas-mediated PCR-free enrichment of the TRPA1 promoter sequence analyzed via nanopore sequencing. Different combinations were tested, with a combination of guide RNA # 4 and # 9 giving the highest number of calls per site. Guide RNAs # 1, # 3 and # 8 have to be assumed to be “interfering”
Guide RNA # | Sequence | PAM | GRCh38 genomic position |
|---|---|---|---|
1 | TTGCCACAAAGAGATCAAGT | AGG | chr.8: 72,070,598 to 72,070,579 |
2 | GAGTATGGTACACCTTCTTG | AGG | chr.8: 72,071,344 to 72,071,363 |
3 | GCACACAACAGAAATGTAGA | AGG | chr.8: 72,070,845 to 72,070,826 |
4 | AGCAATTTTGTGATCCCCTA | AGG | chr.8: 72,070,732 to 72,070,751 |
5 | GAACAAAGACACTCGCTCAA | TGG | chr.8: 72,081,680 to 72,081,661 |
6 | TGGCATGTTAAGACAATTGT | TGG | chr.8: 72,082,102 to 72,082,083 |
7 | GAGCTTCTAATCAGTGACTG | AGG | chr.8: 72,080,169 to 72,080,150 |
8 | ACTTATGCTTACCATTCAGA | TGG | chr.8: 72,080,463 to 72,080,444 |
9 | CCAGTAACATATGAAAAGGT | TGG | chr.8: 72,080,463 to 72,080,444 |