Table 3 Calls per site and methylation rates obtained by application of two different guide RNA strategies. Data obtained by use of guide RNAs # 4 and # 9 are based on four experiments (number of calls per site 55–343), data obtained by usage of all guide RNAs are based on five experiments (number of calls per site 17–51). The same DNA sample extracted from a buffy coat was used for all sequencing experiments

-734

72,076,349

78

343

195

110

91

0

-720

72,076,335

83

328

182

105

92

2

-628

72,076,243

78

273

164

81

39

3

-480

72,076,095

72

313

176

101

09

1

-452

72,076,067

58

243

139

77

32

3

-429

72,076,044

69

252

140

79

60

5

-412

72,076,027

55

281

154

94

25

4

All guide RNAs (# 1 - # 9)

CpG position

Calls per site

Methylation rate (%)

bp relative to exon 1

GRCh38 position

Min

Max

Mean

SD

Mean

SD

-734

72,076,349

26

51

39

10

88

5

-720

72,076,335

23

47

35

10

90

7

-628

72,076,243

18

46

32

11

40

4

-480

72,076,095

20

47

34

10

13

5

-452

72,076,067

18

40

29

7

23

5

-429

72,076,044

19

38

30

9

65

5

-412

72,076,027

17

41

32

12

28

13