Filaggrin gene variants among Saudi patients with ichthyosis vulgaris

BMC Medical Genomics

Table 1 List of primers designed and used to amplify FLG gene

	Name of the primer	Sequence	Amplified PCR product	Annealing temperature
1	FLG501/2282F	AGCCACCAAGAGTCCACACGTGGC	971	72 °C
2	FLG501/2282R	TGATGGTGACCAGCCTGTCCATGGCC	971	72 °C
3	FLG2447F	CGGTCAGCAGGAAGGTCTG	487	65 °C
4	FLG2447R	ATCCCCAGTTCCTGCTTGTC	487	65 °C
5	FLG501F	ACCAAGAGTCCACACGTGGCCG	224	70 °C
6	FLG501R	TCTGCTTGACCCCGGGTGTCCAC	224	70 °C
7	FLG2282F	GACAAGCTTCATCTGCAGTCAGAGA	203	63.6 °C
8	FLG2282R	GTAGAGGAAAGACCCTGAACGTCG	203	63.6 °C

ISSN: 1755-8794