Fig. 3From: Phenotypic variability in two female siblings with oocyte maturation arrest due to a TUBB8 variantTUBB8 expression analysis. (A) Sanger sequencing results revealed that the recombinant plasmid vector was successfully constructed. (B) RT‒qPCR results showed that mutant mRNA expression decreased. (C) WB results showed that the expression levels of mutant TUBB8 protein in HEK293T cells was decreased. ***P < 0.01Back to article page