Table 1 primers used in the experiments
From: Phenotypic variability in two female siblings with oocyte maturation arrest due to a TUBB8 variant
primer | primer sequences | |
|---|---|---|
vector construction | phage-TUBB8-SalI-wt-F | TGACGTCGACaATGAGGGAGATCGTGCTCAC |
phage-TUBB8-SalI-mut-F | TGACGTCGACaATGAGGGAGCTCGTGCTCAC | |
phage-TUBB8-NotI-R | CGACGCGGCCGCTGGCCACCTCCTCCTCGGCAT | |
qPCR | TUBB8-phage-QPCR-F | ATCCACGCTGTTTTGACCTC |
TUBB8-phage-QPCR-R | CGATGGCATGTTCATCAGAG |