Fig. 2

Identification of NAC-related differentially expressed genes and enrichment analysis. A: Volcano plot compares the differentially expressed genes (DEGs), screened by the criteria of |log2FC|>0.585 and p < 0.05, the blue dots denotes the down-regulated DEGs and the red dots denotes the up-regulated DEGs; B: Heatmap of DEGs indicating the expression of the top 50 DEGs in NAC resistant and sensitive groups, each row represents one DEG and each column represents one sample. The red and blue colors represent up-regulated and down-regulated DEGs respectively; C: GO-Biological process (BP) enrichment analysis results of NAC-related DEGs; D: GO-Cellular component (CC) enrichment analysis results of NAC-related DEGs; E: GO-Molecular function (MF) enrichment analysis results of NAC-related DEGs; F: Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis results of NAC-related DEGs, the dot size represents the count of DEGs, and the color depth represents the p-value based significance; G: Expression of KRT7 in different cell clusters; H: Expression of AEBP1 in different cell clusters; I: Expression of BCL2A1 in different cell clusters; J: Expression of RGS1 in different cell clusters; K: AUC histogram for cell activity score of candidate NAC marker genes. The threshold was set as 0.16, and 9297 cells exceeded the threshold; L: The UMAP map is based on the candidate NAC marker genes score of each unit. Cell clusters with high ISG scores are highlighted