Fig. 5From: Identifying potential pathogenesis and immune infiltration in diabetic foot ulcers using bioinformatics and in vitro analysesDual-luciferase reporter assay for validation of miRNA-mRNA regulatory networks. A The three predicted binding sites between miRNA-182-5p or miRNA-338-3p and the 3' untranslated region (3' UTR) of the corresponding genes. B The relative luciferase activity in 293 T cells co-transfected with wild-type (WT) or mutant (MUT) binding site vectors of CHL1, MITF, and NOVA1 3' UTR in the presence of miRNA mimics or a negative control (NC). *𝑃< 0.05, **𝑃< 0.01, ***𝑃< 0.001, ****𝑃< 0.0001Back to article page