Fig. 8
Total RNA m6A modification level and the immune cells infiltration in hepatic tissues among mouse hepatic IRI model. A The total RNA m6A modification level in hepatic tissues were tested by m6A methylation quantification assay. B Representative immunofluorescence staining of F4/80 (marker of macrophages), Ly6G (marker of neutrophils), tryptase (marker of mast cells), CD3 (marker of T lymphocytes) and CD11b (marker of dendritic cells) in hepatic tissues between the post-reperfusion group and control group. Quantification of F4/80, Ly6G, tryptase, CD3 and CD11b per HPF. Scale bar: 100 μm. n = 10 per group, unpaired t test was adopted for statistical analysis. DAPI, 4, 6-diamidino-2-phenylindole; HPF, high power field