Table 1 Forward, reverse, and extension primers of the 4 tag SNPs for PCR
SNPs | Forward and reverse primers (5’-3’) | Extension primers (5’-3’) |
|---|---|---|
rs10754558 | F: 5’-GTGGAGTGTCGGAGAAGAGA-3’ R: 5’-GCTAATTACATGAGGTCACCAAGA-3’ | 5’-CTGACTGACTGACTGACTGACTGACTGCAATGACAGCATCGGGTGTTGTT-3’ |
rs7525979 | F: 5’-CCATCGGCAAGACCAAGAC-3’ R: 5’-CCAGGCTCCTCTGTGTCA-3’ | 5’-CTGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTGTTCTGAGCCTGTGCACAC-3’ |
rs35829419 | F: 5’-CTGTCATCGGGTGGAGTCA-3’ R: 5’-GCGAGGAAGCAGGAGGAA-3’ | 5’-CTGACTGACTGACTGACTGAGAGGAGCTTGGGAGGACACACT-3’ |
rs4612666 | F: 5’-GGTTGCACAACAATGTGAAGT-3’ R: 5’-ATAACAAGTAAGCATTCTCCAAGC-3’ | 5’-TTCTCCAAGCTCCCACCAATACTAC-3’ |