Fig. 7
Relationship between Community 1 and features of ICI responses TME of OSCC (A) The workflow of the GSE153383 dataset. The MOC cells from mouse OSCC line were injected into mice which were subsequently subjected to anti-PD1 therapy. The MOC line produced in responding mice was MOC1, and the MOC line in resistant mice was MOC1esc1. B DEG analysis of MOC1 and MOC1esc1. C Distribution of DEG in Community 1 and non-Community 1. D GSEA analysis on Community 1 in DEG value ranking. E Identification of Community 1-related immune genes in multiple HNSCC cohorts. F Umap plots show immune cell subsets identified through the ProjecTILs algorithm in annotated T cells. G Distribution of immune cell subtypes in different conditions. H Diffusemap shows the distribution of cells in different immune subsets and conditions in dynamic evolution trajectories of CD8 T cells. I Violin plot shows DEGs in CD8 T cells of MOC1 and MOC1esc1. J KEGG analysis of DEGs in CD8 T cells of MOC1 and MOC1esc1. K Diffuse map shows dynamic evolution trajectories of MOC1 CD8 T cells. L Changes in TCR clone size before and after ICI treatment in the dynamic evolution trajectories of MOC1 CD8 T cells. M, N Violin plot and Line plot show DEGs in MOC1 CD8 T cells before and after ICI treatment and their changes in the dynamic evolution trajectories of CD8 T cells. O The identification of iks. P Diffuse map shows the distribution of iks in the dynamic evolution trajectories of CD8 T cells. Q Jaccard similarity coefficient between iks and ProjecTILs immune cell subtypes. R The hill score of iks. S Identification of cell–cell pairs related to Community 1-related immune genes in CellPhoneDB. T Differential cell–cell pairs in Community 1-related immune genes between MOC1(response) and MOC1esc1(resistant)