Fig. 7

Functional analysis of GPX8⁺ CAFs. A t-SNE clustering plot of fibroblasts. Distribution of GPX8 expression in fibroblasts t-SNE plot (B) and violin plot (C). D Heatmap showing enrichment of hallmark gene sets in fibroblast subpopulations. E GSEA analysis for GPX8 ⁺ CAFs. F K-M curve analyzing the prognostic value of GPX8⁺ CAFs in LUAD. G The trajectory plot showed identified fibroblast clusters in pseudo timeline. H Trajectories showing the developmental trajectories of GPX8⁻ CAFs clusters and GPX8⁺ CAFs clusters. I-J Dynamic changes of GPX8 with cell developmental trajectories. K Heatmap of gene expression changes along cellular trajectories