Fig. 2

Relative abundance of sequences after saponin-based host depletion of blood samples. Samples were spiked with 50–100 CFU/mL E. coli and subjected to standard saponin-based depletion method, followed by DNA extraction and nanopore sequencing. All sequenced reads were taxonomically classified. A Relative abundance (%) of human chromosomal or bacterial (any species) DNA in samples after host depletion compared to control samples not subjected to host depletion (B) Relative abundance of human chromosomal and mitochondrial DNA after host depletion compared to control samples not subjected to host depletion. Data are means ± SD. n = 3 are biological replicates