Fig. 3

Host DNA depletion efficiency during methodological developments of saponin concentration, reaction volume and SepsiPURE addition. A Relative quantification of human chromosomal, human mitochondrial and E. coli DNA in blood samples spiked with 50–100 CFU/mL E. coli and subjected to standard (1% saponin final concentration) and increased saponin (3%) depletion protocols. B Relative quantification of human chromosomal, human mitochondrial and E. coli DNA blood samples spiked with 50–100 CFU/mL E. coli and subjected to saponin-based depletion following either the Standard (200µl blood sample) or the Scaled-up (1mL blood sample) protocol. C Relative quantification of human chromosomal, human mitochondrial and E. coli DNA in blood samples spiked with 5–10 CFU/mL E. coli that were subjected to either SepsiPURE protocol only, saponin-based method only and SepsiPURE or saponin-based depletion methods combined. All values were normalised by obtaining their fold change, which represents the difference to an untreated control as measured by qPCR. Data are means, ± SD. A and B n = 4, C n = 3 are biological replicates. ns > 0.05, *p ≤ 0.05, **p ≤ 0.01. ***p ≤ 0.001, ****p ≤ 0.0001