Fig. 4
Amplification efficiency comparison for standard and optimised WGA protocols with E. coli and S. aureus. Different gDNA concentrations equivalent to adding 102–1 CFU of E. coli (A) or S. aureus (B) were subjected to the standard (handbook protocol) and optimised WGA protocols. To compare both methods, samples taken at different time points were ran on qPCR and Ct values were converted to their CFU equivalences. Data are means ± SD. A and B n = 3 are biological replicates. ns > 0.05, *p ≤ 0.05, **p ≤ 0.01. ***p ≤ 0.001, ****p ≤ 0.0001