Fig. 5

Bacterial sequence reads with the 1mL and 5mL CMg pipelines. Number of classified reads obtained on E. coli, S. aureus, K. pneumoniae and E. faecalis when spiked at different concentrations (50–100, 5–10 or 1–5 CFU/mL). All species and concentrations were spiked into 1mL or 5mL of whole blood and were subjected to whole standard and quick-enrichment pipelines. Sequencing results were grouped into two different categories: Target species reads = taxonomically classified reads that matched the spiked species; Other reads = reads that were classified as any other microbial species. Data are means ± SD. n = 3 are biological replicates