Fig. 3

Effects of swimming exercise on the DEGs of the aging lens. (A) The Volcano plot of DEGs. The X axis represented transcript difference (log2-transformed fold changes), and the Y axis represented the corresponding -log10-transformed adj-p values. Red dots indicated significantly up-regulated genes, blue dots denoted significantly down-regulated genes, and gray dots symbolized proteins with no significant change. (B) The bubble map of the overlapping DEGs with previous articles. The green boxes in the first row represent the genes that overlap with Faranda et al., while those in the second row represent the genes that overlap with Zheng et al. The larger the bubbles were with smaller adjusted-p value and greater significance. Red bubbles indicated the relatively up-regulation in AS compared with YS group, and blue bubbles indicated the relatively down-regulation in AE compared with AS group. (C) The hierarchical cluster analysis contained values of the 92 DEGs in YS, AS and AE groups. The red arrows represented the genes that exhibited significant changes in both the AE (vs. AS) and AS groups (vs. YS). (D) Validation of the mRNA expression levels. Data represented mean ± SD. Significant differences between groups were noted by **p < 0.01, ***p < 0.001, ****p < 0.0001. N = 3/group