Table 1 PCR primers, restriction enzymes, and the expected sizes of the amplified and digested fragments of the SNPs associated with TSHR and THRα genes [35]
SNP | PCR product (bp) | Primers | Restriction enzyme | Restricted fragments (bp) |
|---|---|---|---|---|
Rs939348 | 190 | F: 5’-CCTGTGTCTCCCAGCTTAGG-3’ R: 5’-CCACCAGACTCACAGCCTCT-3’ | MesI | C allele190 T allele48 & 142 |
Rs2268458 | 162 | F: 5’-CTAACCAGCAGAGGGAGCAC-3’ R: 5’-CCACTGCTTAAAGCCCAGAT-3’ | AluI | T allele162 C allele 100 & 62 |
Rs2239610 | 172 | F: 5’-CCAGAGATCAAGGGCATCTG-3’ R: 5’-CCAAGTGTGGGCGATTAAGT-3’ | NlaIV | G allele172 C allele142 & 30 |