Table 2 PCR primers, restriction enzymes and the expected size of the amplified and digested fragments of the SNPs associated with DIO2 gene [11]
SNP | PCR product (bp) | Primers | Restriction enzyme | Restricted fragments (bp) |
|---|---|---|---|---|
rs225011 | 323 | F: 5’-CAGCTGCAGAATTCTTTCCA-3’ R: 5’-GGCCCCAGGATTCATATTTC-3’ | XmaJI | TT: 323 CC: 203/120 |
rs225012 | 335 | F: 5’-TGGAGGCTTCTACTGCC ACT-3’ R: 5’-AGGCTGCAAACTTTCCTTCA-3’ | Eam1104I | AA: 335 GG: 243/92 |
rs7140952 | 303 | F: 5’-CCAAAAGAAACCCCTCACAA-3’ R: 5’-GGCAGGTGGAGAGAGTGGTA-3’ | BfmI | TT: 303 CC: 101/202 |
rs2839858 | 370 | F: 5’-TTACCTGCCATCATGCC TCT-3’ R: 5’-GCTCGTGAAAGGAGGTCAAG-3’ | MnlI | AA:158/95/41/46/26/4 CC:204/95/41/46/26/4 |