A novel COL4A5 splicing mutation causes alport syndrome in a Chinese family

BMC Medical Genomics

Table 2 Primers used in the study

Name	Primers	Sequence (5′-3′)	Product length
Round 1 amplification primers for Nested PCR	245,465-COL4A5-F	ctaactggtgtctagctggc	3274 bp
Round 1 amplification primers for Nested PCR	248,738-COL4A5-R	gaatggcccagacaatgaga	3274 bp
Round 2 amplification primers for Nested PCR	245,699-COL4A5-F	catcccagaagcaaatggat	2734 bp
Round 2 amplification primers for Nested PCR	248,432-COL4A5-R	aggctgaggcagaagaattg	2734 bp
pcMINI-N-wt/mut full-length amplification	pcMINI-N-COL4A5-HindIII-F	acttaagcttatgggtccaactggccctccagga	2119 bp
pcMINI-N-wt/mut full-length amplification	pcMINI-N-COL4A5-ECORI-R	tgcagaattctttgatctatgaaccaagat	2119 bp
pcMINI-N mut left half fragment amplification	pcMINI-N-COL4A5-HindIII-F	acttaagcttatgggtccaactggccctccagga	1473 bp
pcMINI-N mut left half fragment amplification	COL4A5-mut-R	cgggtacctatttctacgaaataatcagta	1473 bp
pcMINI-N mut right half fragment amplification	COL4A5-mut-F	tactgattatttcgtagaaataggtacccg	662 bp
pcMINI-N mut right half fragment amplification	pcMINI-N-COL4A5-ECORI-R	tgcagaattctttgatctatgaaccaagat	662 bp

ISSN: 1755-8794