Fig. 2

Validation of microarray results by qRT-PCR. Shown here are log₂ (fold changes) of genes that were determined to be differentially regulated by the 4.45 Gy dose by both dose-rates. The graph on the left shows the mean log₂ (fold change) after LDR 4.45 Gy; and the graph on the right is the mean of log₂ (fold change) after Acute 4.45 Gy exposure. All microarray (blue bars, five biological replicates) and qRT-PCR (red-bars, three biological replicates) results are average fold-change from paired analyses; SEM values for all data are included in Additional file 4