Fig. 1

Intracellular lipid accumulation in undifferentiated, 7- and 21-day differentiated ASCs: a Phase contrast images of cells (10x magnification) are presented in the top panel and the corresponding AdipoRed stained images are shown in the bottom panel. These are representative photographs from two independent experiments each performed in duplicate. b Quantification of intracellular lipid showed significantly increased accumulation of lipid droplets in 21-day differentiated ASCs versus undifferentiated and 7-day differentiated ASCs. Values represent mean ± SEM of two independent experiments performed in triplicate. Statistical analysis was performed using Student’s t test and p < 0.05 was considered significant