Table 8 Breakpoints detected within the AF9 SAR region in BA-treated TWO4 cells

0.5 mM BA, pH 7.4

This breakpoint is identical with a breakpoint identified in NP69 cells treated with BA at pH 7.4. This chromosome break falls within the AF9 region (at coordinates 245,252–245,612) previously reported to be involved in the formation of the MLL-AF9 fusion gene in an ALL patient [GenBank:AM050804]. This breakpoint is three nucleotides different from that reported in cultured normal blood cells treated with VP16 (at coordinate 245,593) [101], five nucleotides different from that detected in H₂O₂-treated NP69 cells (at coordinate 245,591) and six nucleotides different from that identified in H₂O₂-treated HK1 cells (at coordinate 245,590) [97].

This breakpoint is five nucleotides different from that (at coordinate 245,659) identified in NP69 cells treated with H₂O₂ [97]. This chromosome break falls within a repeat ERE2_EH (at coordinates 245,627–245,728).

This chromosome break falls within a repeat ERE2_EH (at coordinates 245,627–245,728).

This breakpoint is identical with that identified in TWO4 cells treated with BA at pH 5.8.

0.5 mM BA, pH 5.8

This chromosome break falls within the AF9 region (at coordinates 245,252–245,612) that was previously reported to translocate with the MLL gene leading to the formation of the MLL-AF9 fusion gene in an ALL patient [GenBank:AM050804].

This chromosome break falls within the AF9 region (at coordinates 245,252–245,612) that was previously reported to translocate with the MLL gene leading to the formation of the MLL-AF9 fusion gene in an ALL patient [GenBank:AM050804].

This chromosome break falls within the AF9 region (at coordinates 245,252–245,612) that was previously reported to translocate with the MLL gene leading to the formation of the MLL-AF9 fusion gene in an ALL patient [GenBank:AM050804]. This breakpoint is one nucleotide different from that reported in cultured normal blood cells treated with VP16 (at coordinate 245,593) [101], three nucleotides different from that detected in H₂O₂-treated NP69 cells (at coordinate 245,591), and four nucleotides different from that identified in H₂O₂-treated HK1 cells (245590) [97].

This breakpoint is identical with the breakpoint previously identified in an ALL patient [GenBank:AM050804].

This chromosome break falls within a repeat ERE2_EH (at coordinates 245,627–245,728).

This breakpoint is identical with a breakpoint identified in TWO4 cells treated with BA at pH 7.4, and seven nucleotides different from that (at coordinate 245,796) reported in NP69 cells treated with H₂O₂ [97].

This breakpoint is six nucleotides different from that (at coordinate 246,000) reported in NP69 cells treated with H₂O₂ [97].

This breakpoint is two nucleotides different from a breakpoint (at coordinate 246,114) reported in CEM cells exposed to VP16 [101], and three nucleotides different from that (at coordinate 246,113) identified in NP69 cells treated with H₂O₂ [97].